Crystallization and preliminary X-ray analysis of an alanine dehydrogenase fromBacillus megateriumWSH-002
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Crystallization and preliminary X-ray analysis of an alanine dehydrogenase from Bacillus megaterium WSH-002. Corrigendum
Alanine dehydrogenase (L-AlaDH) from Bacillus megaterium WSH-002 catalyses the NAD⁺-dependent interconversion of L-alanine and pyruvate. The enzyme was expressed in Escherichia coli BL21 (DE3) cells and purified with a His6 tag by Ni²⁺-chelating affinity chromatography for X-ray crystallographic analysis. Crystals were grown in a solution consisting of 0.1 M HEPES pH 8.0, 12%(w/v) polyethylene ...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section F Structural Biology and Crystallization Communications
سال: 2013
ISSN: 1744-3091
DOI: 10.1107/s1744309113019672